• Lemon
  • Lemon

Lemon

  • Scientific Name
    Citrus limon
  • Family
    Rutaceae
  • Origin
    Italy, US, Argentina, Spain, India, Mexicoetc.

CONTENTS

Plant description

Lemon belongs to the Rutaceae family and grows up to around 4 meters.  It has small white flowers all year round, green leaves, and its branches have distinctive thorns. Some varieties without thorns are available but this is due to breeding efforts. The fruit of the lemon is renowned for its distinctive oval shape, vibrant yellow color, and yellow flesh. Among citrus fruits, lemons are notably high in acidity and vitamin C content. The name “lemon” is believed to have originated from the Arabic word “laimun,” which refers to citrus fruit, as well as the Persian word “limun.”The essential oil is primarily found in the oil sacs of the lemon peel. While lemons originally hail from India, they are now cultivated extensively around the world, with major producers including India, Mexico, and China.

Essential oil composition

Lemon (cold-pressed)

  • Lemon (cold-pressed)

  • INCI
    CITRUS LIMON (LEMON) PEEL OIL
  • Parts of Plant Used
    Peel
  • Extraction Method
    Cold Press
  • Extraction Rate
    0.3 - 0.4%
  • Notes
    Top
  • Aroma Family
    Citrus

Lemon (cold-pressed) Essential Oil Properties & Uses

About 0.3 - 0.4% of Lemon essential oil is obtained through Cold Press from its peel and is colorless to pale yellow. Its primary components, limonene and citral, impart a delightful citrus aroma. It's important to note that it contains furocoumarins, particularly bergapten, which can cause phototoxic reactions. It has a clear, clean, and invigorating aroma, with a refreshing lemon-like acidity and a hint of bitterness. It blends harmoniously with various other essential oils, but its fragrance might appear subtler than others. When blending, careful consideration of the strength and quantity is necessary to achieve a balanced aroma. Given its relatively mild scent, adding a bit more to the blend can help maintain a well-balanced scent. This versatile oil is used in food flavors, cosmetic fragrances, diffusers, and detergents.

Essential oil composition

Essential oil composition

※An example of one batch production is shown here.

  • Limonene　68.44%
  • β-Pinene　12.40%
  • γ-Terpinene　8.54%
  • α-Pinene　2.02%
  • Sabinene　1.68%
  • Myrcene　1.60%
  • Citral　1.45%
  • p-Cymene　1.07%
  • Decanal 0.01%
  • Other components　2.79%
Lemon (furocoumarin-free)

  • Lemon (furocoumarin-free)

  • INCI
    CITRUS LIMON (LEMON) PEEL OIL
  • Parts of Plant Used
    Peel
  • Extraction Method
    Cold Press
  • Extraction Rate
    0.3 - 0.4%
  • Notes
    Top
  • Aroma Family
    Citrus

Lemon (furocoumarin-free) Essential Oil Properties & Uses

About 0.3 - 0.4% of Lemon essential oil is obtained through Cold Press from its peel and is colorless to pale yellow. Its primary components, limonene and citral, impart a delightful citrus aroma. It has a clear, clean, and invigorating aroma, with a refreshing lemon-like acidity and a hint of bitterness. When furocoumarins are removed, resulting in a phototoxic ity-free fragrance, the scent becomes slightly lighter compared to regular pressed oils. Therefore, for aromatic purposes, Cold Press is recommended. It blends harmoniously with various other essential oils, but its fragrance might appear subtler than others. When blending, careful consideration of the strength and quantity is necessary to achieve a balanced aroma. Given its relatively mild scent, adding a bit more to the blend can help maintain a well-balanced scent. This versatile oil is used in food flavors, cosmetic fragrances, diffusers, and detergents.

Essential oil composition

Essential oil composition

※An example of one batch production is shown here.

  • Limonene　68.44%
  • β-Pinene　12.40%
  • γ-Terpinene　8.54%
  • α-Pinene　2.02%
  • Sabinene　1.68%
  • Myrcene　1.60%
  • Citral　1.45%
  • p-Cymene　1.07%
  • Decanal 0.01%
  • Other components　2.79%

Functions and Efficacies

α-Pinene

α-Pinene
  • IUPAC Name
    (+)-α-Pinene: (1R,5R)-2,6,6-trimethylbicyclo[3.1.1]hept-2-ene
    (-)-α-Pinene: (1S,5S)-2,6,6-trimethylbicyclo[3.1.1]hept-2-ene
  • Molecular Formula
    C10H16
  • Molecular Weight
    136.23 g/mol
  • CAS No.
    (+)-α-Pinene: 7785-70-8
    (-)-α-Pinene: 7785-26-4
  • Structure
    Bicyclic monoterpene

Functions and Efficacies of α-Pinene

in slico in vitro ex vivo in vivo
Non-clinical Clinical
Mice Rats Guinea Pigs Insects Rabbits Human
Stress Reduction ○
Anti-bacterial Effects ○
Anti-inflammatory Effects ○

Stress Reduction

A study reported that brain tumors in mice that inhaled the fragrance of α-pinene became smaller. However, no melanoma cell growth was observed with the addition of α-pinene, indicating a potential psychological effect .1)

Anti-bacterial Effects

A study reported that α-pinene had an inhibitory effect on the growth of blue staining fungi, both when exposed to gas and when added to the culture medium2)

Anti-inflammatory Effects

It is known that the addition of lipopolysaccharide (LPS) in RAW264.7 cells induces the production of nitric oxide (NO), a substance that causes inflammation. A study reported that the production of NO was suppressed by the addition of α-pinene.3)

  • 1)Kusuhara et al., Biomedical Research, 2012
  • 2)岡村、木材保存、
  • 3)Kwak et al., Journal of Exercise Rehabilitation, 2019

Citral

Citral
  • IUPAC Name
    (2E)-3,7-dimethylocta-2,6-dienal
  • Molecular Formula
    C10H16O
  • Molecular Weight
    152.23 g/mol
  • CAS No.
    5392-40-5
  • Structure
    Monoterpene

Functions and Efficacies of Citral

in slico in vitro ex vivo in vivo
Non-clinical Clinical
Mice Rats Guinea Pigs Insects Rabbits Human
Stress Reduction ○
Anti-bacterial Effects ○
Anti-cancer Effects ○

Stress Reduction

A study reported that mice exposed to cold stress at 4°C exhibited suppressed increases in brain monoamine levels within the citral-treated group.1)

Acti-bacterial Effects

A study reported that when Aspergillus niger was cultivated in a growth medium supplemented with citral, it exhibited superior antimicrobial activity compared to other essential oil components. Additionally, citral demonstrated a longer-lasting antimicrobial effect against multiple fungi.2)

Anti-cancer Effects

A study reported that the administration of citral to human breast cancer-derived 3D-cultured cells resulted in a concentration-dependent reduction in the size of cell aggregates. Additionally, in experiments utilizing AnnexinV/7AAD flow cytometry—a method used to detect apoptotic cells—an increase in apoptotic cells was observed following citral administration.3)

  • 1)Fukumoto et al., Stress and Health, 2008
  • 2)岡村、木材保存、2002
  • 3)Nigjeh et al., BMC Complementary and Alternative Medicine, 2018

Limonene

Limonene
  • IUPAC Name
    1-methyl-4-prop-1-en-2-ylcyclohexene
  • Molecular Formula
    C10H16
  • Molecular Weight
    136.23 g/mol
  • CAS No.
    138-86-3
  • Structure
    Monocyclic monoterpene

Functions and Efficacies of Limonene

in slico in vitro ex vivo in vivo
Non-clinical Clinical
Mice Rats Guinea Pigs Insects Rabbits Human
Stress Reduction ○
Memory Improvement ○ ○
Anti-cancer Effects ○ ○ ○

Stress Reduction

A study reported that mice subjected to cold stress at 4°C (41°F) exhibited reduced elevations in blood corticosterone levels when treated with limonene. Furthermore, the same report demonstrated that limonene effectively lowered corticosterone levels in the blood of mice exposed to both physical and mental stress.1)

Memory Improvement

A study reported that when acetylcholinesterase reacts with acetylthiocholine in phosphate-buffered saline, the introduction of limonene inhibits the degradation by acetylcholinesterase. Acetylcholinesterase plays a role in the breakdown of acetylcholine, a hormone associated with memory and learning. In the same report, it was also noted that limonene suppressed memory impairment induced by scopolamine administration in rats.2)

Anti-cancer Effects

A report indicated that the consumption of limonene by breast cancer patients resulted in reduced expression of Cyclin D1, a protein crucial for cell division, within tumors.3)Furthermore, numerous other studies have also documented the anticancer properties of limonene.4)5)

  • 1)Fukumoto et al., Stress and Health, 2008
  • 2)Zhou et al., Nutritional Neuroscience,, 2013
  • 3)Miller et al., Cancer Prevntion Research, 2013
  • 4)Ajikumaran Nair S et al., Phytomedicine, 2018
  • 5)Chaudhary et al., Human & Experimental Toxicology, 2012

α-Pinene

α-Pinene
  • IUPAC Name
    (+)-α-Pinene: (1R,5R)-2,6,6-trimethylbicyclo[3.1.1]hept-2-ene
    (-)-α-Pinene: (1S,5S)-2,6,6-trimethylbicyclo[3.1.1]hept-2-ene
  • Molecular Formula
    C10H16
  • Molecular Weight
    136.23 g/mol
  • CAS No.
    (+)-α-Pinene: 7785-70-8
    (-)-α-Pinene: 7785-26-4
  • Structure
    Bicyclic monoterpene

Functions and Efficacies of α-Pinene

in slico in vitro ex vivo in vivo
Non-clinical Clinical
Mice Rats Guinea Pigs Insects Rabbits Human
Stress Reduction ○
Anti-bacterial Effects ○
Anti-inflammatory Effects ○

Stress Reduction

A study reported that brain tumors in mice that inhaled the fragrance of α-pinene became smaller. However, no melanoma cell growth was observed with the addition of α-pinene, indicating a potential psychological effect .1)

Anti-bacterial Effects

A study reported that α-pinene had an inhibitory effect on the growth of blue staining fungi, both when exposed to gas and when added to the culture medium2)

Anti-inflammatory Effects

It is known that the addition of lipopolysaccharide (LPS) in RAW264.7 cells induces the production of nitric oxide (NO), a substance that causes inflammation. A study reported that the production of NO was suppressed by the addition of α-pinene.3)

  • 1)Kusuhara et al., Biomedical Research, 2012
  • 2)岡村、木材保存、
  • 3)Kwak et al., Journal of Exercise Rehabilitation, 2019

Citral

Citral
  • IUPAC Name
    (2E)-3,7-dimethylocta-2,6-dienal
  • Molecular Formula
    C10H16O
  • Molecular Weight
    152.23 g/mol
  • CAS No.
    5392-40-5
  • Structure
    Monoterpene

Functions and Efficacies of Citral

in slico in vitro ex vivo in vivo
Non-clinical Clinical
Mice Rats Guinea Pigs Insects Rabbits Human
Stress Reduction ○
Anti-bacterial Effects ○
Anti-cancer Effects ○

Stress Reduction

A study reported that mice exposed to cold stress at 4°C exhibited suppressed increases in brain monoamine levels within the citral-treated group.1)

Acti-bacterial Effects

A study reported that when Aspergillus niger was cultivated in a growth medium supplemented with citral, it exhibited superior antimicrobial activity compared to other essential oil components. Additionally, citral demonstrated a longer-lasting antimicrobial effect against multiple fungi.2)

Anti-cancer Effects

A study reported that the administration of citral to human breast cancer-derived 3D-cultured cells resulted in a concentration-dependent reduction in the size of cell aggregates. Additionally, in experiments utilizing AnnexinV/7AAD flow cytometry—a method used to detect apoptotic cells—an increase in apoptotic cells was observed following citral administration.3)

  • 1)Fukumoto et al., Stress and Health, 2008
  • 2)岡村、木材保存、2002
  • 3)Nigjeh et al., BMC Complementary and Alternative Medicine, 2018

Limonene

Limonene
  • IUPAC Name
    1-methyl-4-prop-1-en-2-ylcyclohexene
  • Molecular Formula
    C10H16
  • Molecular Weight
    136.23 g/mol
  • CAS No.
    138-86-3
  • Structure
    Monocyclic monoterpene

Functions and Efficacies of Limonene

in slico in vitro ex vivo in vivo
Non-clinical Clinical
Mice Rats Guinea Pigs Insects Rabbits Human
Stress Reduction ○
Memory Improvement ○ ○
Anti-cancer Effects ○ ○ ○

Stress Reduction

A study reported that mice subjected to cold stress at 4°C (41°F) exhibited reduced elevations in blood corticosterone levels when treated with limonene. Furthermore, the same report demonstrated that limonene effectively lowered corticosterone levels in the blood of mice exposed to both physical and mental stress.1)

Memory Improvement

A study reported that when acetylcholinesterase reacts with acetylthiocholine in phosphate-buffered saline, the introduction of limonene inhibits the degradation by acetylcholinesterase. Acetylcholinesterase plays a role in the breakdown of acetylcholine, a hormone associated with memory and learning. In the same report, it was also noted that limonene suppressed memory impairment induced by scopolamine administration in rats.2)

Anti-cancer Effects

A report indicated that the consumption of limonene by breast cancer patients resulted in reduced expression of Cyclin D1, a protein crucial for cell division, within tumors.3)Furthermore, numerous other studies have also documented the anticancer properties of limonene.4)5)

  • 1)Fukumoto et al., Stress and Health, 2008
  • 2)Zhou et al., Nutritional Neuroscience,, 2013
  • 3)Miller et al., Cancer Prevntion Research, 2013
  • 4)Ajikumaran Nair S et al., Phytomedicine, 2018
  • 5)Chaudhary et al., Human & Experimental Toxicology, 2012

IFRA

Lemon：
There are restrictions on its use in the final product due to the possibility of phototoxicity caused by the presence of furocoumarins (bergapten).

Lemon

※Only information for selected components are shown here.

Limonene：
Oxidized limonene can cause dermal sensitization. For essential oils with a high limonene content, it is advisable to use only when the level of peroxides is maintained at a minimum (20 mol/l) through the incorporation of antioxidants.

※Only information for selected components are shown here.

Limonene：
Oxidized limonene can cause dermal sensitization. For essential oils with a high limonene content, it is advisable to use only when the level of peroxides is maintained at a minimum (20 mol/l) through the incorporation of antioxidants.

※Only information for selected components are shown here.

References
Jennie Harding [Handbook of Essential Oil and Plant Oil] Tokyo-do Publishing, 2010.
Shinichiro Otsuki/Yukiko Ozaki [Scientific Name Etymology Dictionary of Herbs] Tokyo-do Publishing, 2009.
Jennie Harding [Illustrated Reference Book of Herbs] Sancho Publishing, 2012.
Freddy Ghozland/Xavier Fernandez [L’Herbier Parfume] Hara Publishing, 2013.
Kyohei Mikami [Comprehensive Guide to Essential Oils] Fragrance Journal, 2010.
Yuzuru Ogura [Dictionary of Plants] Tokyo-do Publishing, 1957.
Nobuo Ohashi [Dictionary of Medical Herbs] Tokyo-do Publishing, 2016.
Wanda Sellar [The directory of essential oils] Fragrance Journal, 1992.
Aroma Environment Association of Japan [AEAJ Aromatherapy Certification Official Textbook grade1&2] Sekaibunka, 1999.
Robert Tissearand [Essential Oil Safety] Fragrance Journal, 2018.
Ayako Berg [Aromatherapy Essential Oil Dictionary] Seibido Shuppan, 2022.
Azusa Annells [Enjoying Fragrance: An Illustrated Book of Aromas with Characteristics]Natsumesha CO.,LTD., 2023
International Fragrance Association, Lemon oil cold pressed, 2020.
International Fragrance Association, Limonene, 1995.
(All written in or translated to Japanese.)
Supervisor
Yamamoto Perfumery co., ltd.
References
Jennie Harding [Handbook of Essential Oil and Plant Oil] Tokyo-do Publishing, 2010.
Shinichiro Otsuki/Yukiko Ozaki [Scientific Name Etymology Dictionary of Herbs] Tokyo-do Publishing, 2009.
Jennie Harding [Illustrated Reference Book of Herbs] Sancho Publishing, 2012.
Freddy Ghozland/Xavier Fernandez [L’Herbier Parfume] Hara Publishing, 2013.
Kyohei Mikami [Comprehensive Guide to Essential Oils] Fragrance Journal, 2010.
Yuzuru Ogura [Dictionary of Plants] Tokyo-do Publishing, 1957.
Nobuo Ohashi [Dictionary of Medical Herbs] Tokyo-do Publishing, 2016.
Wanda Sellar [The directory of essential oils] Fragrance Journal, 1992.
Aroma Environment Association of Japan [AEAJ Aromatherapy Certification Official Textbook grade1&2] Sekaibunka, 1999.
Robert Tissearand [Essential Oil Safety] Fragrance Journal, 2018.
Ayako Berg [Aromatherapy Essential Oil Dictionary] Seibido Shuppan, 2022.
Azusa Annells [Enjoying Fragrance: An Illustrated Book of Aromas with Characteristics]Natsumesha CO.,LTD., 2023
International Fragrance Association, Limonene, 1995.
(All written in or translated to Japanese.)
Supervisor
Yamamoto Perfumery co., ltd.

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